دانلود کتاب Methods for General and Molecular Microbiology

This book will be an invaluable resource to every serious practitioner of microbiology in academia, industry, and government, and in clinical laboratories for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. New chapters cover important topics such as molecular genetics of archaea; molecular genetic manipulations using phages as tools; state-of-the-art technology used in studying complex, naturally occurring microbial communities; metabolism of natural polymers including cellulose, hemicellulose, and lignin; DNA microarray technology; physiology, metabolism, molecular genetic approaches to the study of filamentous fungi, and general and molecular approaches for studying microbial symbioses. Each section details reliable methods applicable to basic studies. Additionally, chapters present background principles to facilitate understanding of the how and why of a given method, followed by a step-by-step description of the procedure. Common problems, precautions, and pitfalls of the methods are also presented as well as commercial sources for equipment and materials. Content: Front Matter • Preface • Table of Contents •Section 1. Morphology and Ultrastructure• Introduction to Morphology and Ultrastructure 1. Light Microscopy 2. Sampling and Staining for Light Microscopy 3. Laser Scanning Microscopy 4. Electron Microscopy 5. Computational Image Analysis and Reconstruction from Transmission Electron Micrographs 6. Atomic Force Microscopy 7. Cell Fractionation 8. Antigen-Antibody Reactions •Section 2. Growth Introduction to Growth 9. Growth Measurement 10. Nutrition and Media 11. Enrichment and Isolation 12. Culture Techniques 13. Energetics, Stoichiometry, and Kinetics of Microbial Growth 14. Physicochemical Factors in Growth 15. Phenotypic Characterization and the Principles of Comparative Systematics 16. General Methods to Investigate Microbial Symbioses •Section 3. Metabolism• Introduction to Metabolism 17. Physical Analysis and Purification Methods 18. Chemical Analysis 19. Enzymatic Activity 20. Permeability and Transport 21. Bacterial Respiration 22. Carbohydrate Fermentations 23. Metabolism of Aromatic Compounds 24. Cellulases, Hemicellulases, and Pectinases 25. Lignin and Lignin-Modifying Enzymes •Section 4. Molecular Genetics• Introduction to Molecular Genetics 26. Similarity Analysis of DNAs 27. Nucleic Acid Analysis 28. Measuring Spontaneous Mutation Rates 29. Transposon Mutagenesis 30. Plasmids 31. Gene Transfer in Gram-Negative Bacteria 32. Genetic Exchange in Gram-Positive Bacteria 33. Genetics of Archaea 34. Genetic Manipulations Using Phages •Section 5. Community and Genomic Analysis Introduction to Community and Genomic Analysis 35. Characterization of Bacterial Genome Sequences by Similarity Searching 36. Reconstructing and Interpreting Evolutionary Relationships 37. Microbial Nucleotide Fingerprints in Nature 38. Construction of BAC and Fosmid Libraries from Naturally Occurring Microbial Populations 39. Single Cell Identification by Fluorescence in situ Hybridization 40. Measurement of rRNA Abundance by Hybridization with Oligodeoxynucleotide Probes 41. Analysis of Microbial Communities with Denaturing Gradient Gel Electrophoresis and Terminal Restriction Fragment Length Polymorphism •Section 6. Mycology Introduction to Mycology 42. Methods for Studying Terrestrial Fungal Ecology and Diversity 43. Physiology Metabolism and Molecular Aspects of Filamentous Fungi 44. Microbiological and Genetic Methods for Filamentous Fungi 45. Principles and Practice of DNA Microarray Technology Appendices Index • Color Plate