دانلود کتاب Clinical Diabetes Research: Methods and Techniques

A practical "how to" guide for multiple methods in metabolism, with a critical and objective discussion of strengths, limitations, and appropriate applications of the described methods.Edited by the winner of the Oskar-Minkowski Prize of the EASD in 2006 Clinical trials in populations at risk of or with overt diabetes mellitus are being performed all around the world to test novel drugs and approaches to managing these diseases. During the last decade, new methods and techniques have been introduced – and are being developed further – that facilitate monitoring of metabolism and even diagnosis of certain defects in human metabolism. While some of these methods are restricted to research facilities, others are already used in clinical practice. The correct beneficial use of these methods requires knowledge of the nature of the techniques and their clinical relevance. Clinical Diabetes Research: Methods and Techniques provides summaries of established state-of-the art-techniques and introduces the new technologies, evaluating their potential in clinical diabetes research. One section compares the efficacy of all metabolic tests that measure insulin action. Another section describes methods that allow quantification of whole body and intracellular metabolic fluxes relevant to diabetes research. The final section focuses on the planning and pitfalls of clinical studies in the field of metabolism. Each chapter throughout the book combines the theoretical basis of the approach with practical recommendations. There are further chapters on the design of clinical trials and ethical considerations. The publication is timely in view of the impressive development of the methods and techniques with their application to understanding the pathophysiology of diabetes, insulin resistance syndromes, obesity, and the metabolic effects of treatment. Clinical Diabetes Research: Methods and Techniques is of great relevance for researchers (from students and post-docs to principal investigators) in the field of human metabolism, clinical endocrinologists and diabetologists who are involved in clinical studies.Content: Chapter 1 Basics of Clinical Metabolic Research (pages 1–5): Michael RodenChapter 2 Methods for the Assessment of ??Cell Function In Vivo (pages 7–26): Andrea Mari and Giovanni PaciniChapter 3 Assessment of Insulin Sensitivity from Steady?State and Dynamic Tests (pages 27–41): Giovanni Pacini and Andrea MariChapter 4 Glucose Clamp Techniques (pages 43–76): Attila Brehm and Michael RodenChapter 5 Methods of Assessment of Counterregulation to Hypoglycaemia (pages 77–103): Pratik Choudhary, Ming Ming Teh and Stephanie A. AmielChapter 6 Glucose Kinetics: Measurement of Flux Rates (pages 105–124): Jerry Radziuk and Susan PyeChapter 7 Xenobiotics as Probes of Carbohydrate Metabolism (pages 125–137): Bernard LandauChapter 8 Tracing Hepatic Glucose and Glycogen Fluxes with 2H2O (pages 139–149): John G. JonesChapter 9 Lipid Kinetics (pages 151–168): John M. Miles and Robert H. NelsonChapter 10 Protein and Amino Acid Kinetics (pages 169–191): Stephen F. Previs, Danielle A. Gilge and Nadia RachdaouiChapter 11 Assessment of Metabolic Fluxes by In Vivo MR Spectroscopy (pages 193–222): Martin Krssak and Michael RodenChapter 12 Positron Emission Tomography in Metabolic Research (pages 223–235): Pirjo Nuutila, Patricia Iozzo and Juhani KnuutiChapter 13 Assessment of Body Fat Content and Distribution (pages 237–263): Martin KrssakChapter 14 Tissue Biopsies in Diabetes Research (pages 265–288): Kurt Hojlund, Michael Gaster and Henning Beck?NielsenChapter 15 Assessment of Vascular Function (pages 289–310): Johannes Pleiner and Michael WolztChapter 16 Cardiovascular Autonomic Function Testing (pages 311–324): Dan ZieglerChapter 17 Nerve Function Testing (pages 325–340): Haris M. Rathur and Andrew J. M. BoultonChapter 18 Kidney Function (pages 341–355): Sally M. MarshallChapter 19 Techniques for the Investigation of the Eye in Diabetes (pages 357–366): Ayad Al?Bermani and Roy TaylorChapter 20 Basics of Molecular Genetics: Lessons from Type 2 Diabetes (pages 367–376): Leif Groop and Charlotte LingChapter 21 Good Clinical Practice: Friend or Foe? (pages 377–385): Christian Joukhadar and Markus MullerChapter 22 Statistical Considerations in Diabetes Trials (pages 387–393): Irene M. Stratton and Carole A. Cull