جزییات کتاب
New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems. For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli. Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study This volume provides, at research and professional level, an overview of our current understanding of the significance of protein-protein interactions in plant biology. Protein-protein interactions in plant thrioredoxin dependent systems / Yve Meter, Myroslawa Miginiac-Maslow, Peter Schurmenn and Jean-Pierre Jacquot -- The Rubisco activase-ruisco system: an ATPase-dependet association that regulates photosynthesis / Archie R. Portis Jr. -- 14-3-3 protein: effectors of enzyme function / Paul C. Sehnke and Robert J. Ferl -- Proteinase inhibitors / William laing and Miachel T. McManus -- Multienzyme complexes involved in the Benson-Calvin cycle and in fatty acid metabolism / Brigitte Gontero, Sandrine Lebreton and Emmanuelle Graciet -- Self-compartmentalizaing proteolytic complexes / Bjarke Veierskov and Christina Ingvardsen -- The higher plant chaperonins / Rajch Sharkia, Paul Viitanen, Galit Levy-Rimler, Celeste Weiss, Adina Niv and Abdussalam Azem -- Receptro kinases / Andrew C. Allan and Keith Hudson -- Cytoplasmatic protein kinases in signal transduction / Claudia Jonak and Heribert Hirt -- Protein-protein interactions in the regulation of plant gene trascription / Dao-Xiu Zhou -- Calmodulin / Teerapong Buaboocha and Raymond E. Zielinski