دانلود کتاب Essential zebrafish methods : cell and developmental biology

The methods and techniques in this volume were chosen by the editors of 'Methods in Cell Biology', whose goal was to provide fail-safe methods, tips, and 'tricks of the trade' to experienced researchers and more junior members in the lab. Content: Overview of the zebrafish system / H. William Detrich, Monte Westerfield, and Leonard I. Zon -- Cell cycles and development in the embryonic zebrafish / Donald A. Kane -- Primary fibroblast cell culture / Barry H. Paw and Leonard I. Zon -- Production of haploid and diploid androgenetic zebrafish (including methodology for delayed in vitro fertilization) / Graham E. Corley-Smith ... [et al.] -- Analysis of protein and gene expression / Trevor Jowett -- Analysis of zebrafish development using explant culture assays / Yevgenya Grinblat and Hazel Sive -- Confocal microscopic analysis of morphogenetic movements / Mark S. Cooper, Leonard A. D'Amico, and Clarissa A. Henry -- Cytoskeletal dynamics of the zebrafish embryo / Jacek Topczewski and Lilianna Solnic-Krezel -- Analyzing axon guidance in the zebrafish retinotectal system / Lara D. Hutson, Douglas S. Campbell, and Chi-Bin Chien -- Analysis of cell proliferation, senescence, and cell death in zebrafish embryos / Daniel Verduzco and James F. Amatruda -- Cellular dissection of zebrafish hematopoiesis / David L. Stachura and David Traver -- Culture of embryonic stem and primordial germ cell lines from zebrafish / Ten-Tsao Wong, Lianchun Fan, and Paul Collodi -- Neurogenesis / Prisca Chapouton, Marion Coolen, and Laure Bally-Cuif -- Time-lapse microscopy of brain development / Reinhard W. Köster and Scott E. Fraser -- Development of the peripheral sympathetic nervous system in zebrafish / Rodney A. Stewart ... [et al.] -- Approaches to study the zebrafish retina / Jarema Malicki and Andrei Avanesov -- Instrumentation for measuring oculomotor performance and plasticity in larval organisms / James C. Beck ... [et al.] -- Development of cartilege and bone / Yashar Javidan, Courtney Alexander, and Thomas F. Schilling -- Morphogenesis of the jaw : development beyond the embryo / R. Craig Albertson and Pamela C. Yelick -- Cardiac development / Le A. Trinh and Didier Y.R. Stainier -- Zebrafish kidney development / Iain A. Drummond. Abstract: Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. This book provides overview of commonly used methods and a comprehensive collection of protocols describing the powerful techniques. Read more...