دانلود کتاب Alternative pre-mRNA Splicing: Theory and Protocols

The book consists of two major parts: The first one provides a brief theoreticalintroduction that gives a short overview of alternative splicing and cites key papers in the field for more in-depth information. The second part is a collection of experimental protocols that are used in the field of alternative splicing.Contents1 Splicing in the RNA World2 RNPs, Small RNAs, and miRNAs3 RNA Elements Involved in Splicing4 A Structural Biology Perspective of Proteins Involved in Splicing Regulation5 The Spliceosome in Constitutive Splicing6 The Use of Saccharomyces cerevisiae to Study the Mechanism of pre-mRNA Splicing7 Challenges in Plant Alternative Splicing8 Alternative Splice Site Selection9 Integration of Splicing with Nuclear and Cellular Events10 Splicing and Disease11 From Bedside to Bench: How to Analyze a Splicing Mutation12 Analysis of Common Splicing Problems13 Ultracentrifugation in the Analysis and Purification of Spliceosomes Assembled In Vitro14 Chemical Synthesis of RNA15 RNA Interference (siRNA, shRNA)16 Expression and Purification of Splicing Proteins17 Detection of RNA–Protein Complexes by Electrophoretic Mobility Shift Assay18 Functional Analysis of Large Exonic Sequences Through Iterative In Vivo Selection19 Identification of Splicing cis-Elements Through an Ultra-Refined Antisense Microwalk20 Genomic SELEX to Identify RNA Targets of Plant RNA-Binding Proteins21 Quantification of Alternative Splice Variants22 High-Throughput Analysis of Alternative Splicing by RT-PCR23 Monitoring Changes in Plant Alternative Splicing Events24 Array Analysis25 The CLIP Method to Study Protein–RNA Interactions in Intact Cells and Tissues26 RNA–Protein Crosslinking and Immunoprecipitation (CLIP) in Schizosaccharomyces pombe27 Identification of Proteins Bound to RNA28 Single-Cell Detection of Splicing Events with Fluorescent Splicing Reporters29 The Preparation of HeLa Cell Nuclear Extracts30 In Vitro Splicing Assays31 Assembly and Isolation of Spliceosomal Complexes In Vitro32 Analysis of Site-Specific RNA–Protein Interactions33 Immunoprecipitation and Pull-Down of Nuclear Proteins34 Analysis of Protein (-RNA) Complexes by (Quantitative) Mass Spectrometric Analysis35 Fast Cloning of Splicing Reporter Minigenes36 In Vivo Analysis of Splicing Assays37 Coupled Promoter Splicing Systems38 Stable Cell Lines with Splicing Reporters39 Splicing Factor ChIP and ChRIP: Detection ofSplicing and Splicing Factors atGenes by Chromatin Immunoprecipitation40 Yeast Genetics to Investigate the Function of Core Pre-mRNA Splicing Factors41 Analysis of HIV-1 RNA Splicing42 In Vivo Analysis of Plant Intron Splicing43 Modification State-Specific Antibodies44 Analysis of Alternative Splicing in Drosophila Genetic Mosaics45 Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing46 Screening for Alternative Splicing Modulators47 Use of Oligonucleotides to Change Splicing48 Changing Signals to the Spliceosome49 Overview of Splicing Relevant Databases50 Analysis of RNA Transcripts by High-Throughput RNA Sequencing51 Identification of Splicing Factor Target Genes by High-Throughput Sequencing52 Bioinformatic Analysis of Splicing Events53 Analysis of Pre-mRNA Secondary Structures and Alternative Splicing54 Structure Prediction for Alternatively Spliced Proteins55 Comparative Genomics Methods for the Prediction of Small RNA-Binding Sites