دانلود کتاب G proteins : techniques of analysis

G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility. G Proteins: Techniques of Analysis includes expression and functions analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and mapping pathways established among receptors, G proteins, and effectors. Content: Expression and purification of G protein [alpha] subunits in Escherichia coli / Maurine E. Linder -- Purification of recombinant G protein [alpha] and [beta][gamma] subunits from Sf9 cells / Tohru Kozasa -- Expression and functional analysis of G protein [alpha] subunits in mammalian cells / Catherine H. Berlot -- Identification of regulators and targets of G protein [alpha] subunits using the yeast two-hybrid system / Jennifer L. Glick, Thomas E. Meigs, and Patrick J. Casey -- Functional analysis of G protein [beta][gamma] subunits / K. Yan ... [et al.] -- Immunological techniques of G protein analysis / Catherine A. Chen and David R. Manning -- GTPase-activating proteins (GAPs) for heterotrimeric G proteins / Jun Wang ... [et al.] -- Fatty acid acylation of G protein [alpha] subunits / Philip B. Wedegaertner -- ADP-ribosylation of G proteins with cholera toxin or pertussis toxin / Marilyn J. Woolkalis -- Analysis of receptor-G protein coupling with pertussis toxin-resistant mutants of Gi and Go / Albrecht Moritz and Ronald Taussig -- Microinjection of antibodies neutralizing G protein function / Vickie J. LaMorte, James R. Feramisco, and Judy L. Meinkoth -- Agonist-promoted (³⁵S)GTP[gamma]S-binding as a probe of receptor-G protein communication in reconstituted Sf9 cells / Alastair J. Barr and David R. Manning -- Methods of sequestering [beta][gamma] in the intact cell / Gregory J. Della Rocca, Robert J. Lefkowitz, and Walter J. Koch -- In vitro and in vivo analysis of heterotrimeric G-protein signaling via DNA/RNA antisense strategies / Hsien-yu Wang ... [et al.] Abstract: G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility. G Proteins: Techniques of Analysis includes expression and functions analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and mapping pathways established among receptors, G proteins, and effectors