دانلود کتاب Protein Engineering Handbook, Volume 1 & Volume 2

Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas. Content: Chapter 1 Guidelines for the Functional Analysis of Engineered and Mutant Enzymes (pages 1–13): Dale E. Edmondson and Giovanni GaddaChapter 2 Engineering Enantioselectivity in Enzyme?Catalyzed Reactions (pages 15–46): Romas KazlauskasChapter 3 Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes (pages 47–79): Stefanie Jonas and Florian HollfelderChapter 4 ??Value Analysis of Protein Folding Transition States (pages 81–120): Neil Ferguson and Alan R. FershtChapter 5 Protein Folding and Solubility: Pathways and High?Throughput Assays (pages 121–145): Adam C. Fisher, Thomas J. Mansell and Matthew P. DeLisaChapter 6 Protein Dynamics and the Evolution of Novel Protein Function (pages 147–185): Jorg Zimmermann, Megan C. Thielges, Wayne Yu and Floyd E. RomesbergChapter 7 Gaining Insight into Enzyme Function through Correlation with Protein Motions (pages 187–211): Nicolas Doucet and Joelle N. PelletierChapter 8 Structural Frameworks Suitable for Engineering (pages 213–232): Birte HockerChapter 9 Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space (pages 233–269): Ana Beloqui, Miren Zumarraga, Miguel Alcalde, Peter N. Golyshin and Manuel FerrerChapter 10 Inteins in Protein Engineering (pages 271–293): Alison R. Gillies and David W. WoodChapter 11 From Prospecting to Product – Industrial Metagenomics Is Coming of Age (pages 295–323): Jurgen Eck, Esther Gabor, Klaus Liebeton, Guido Meurer and Frank NiehausChapter 12 Computational Protein Design (pages 325–342): Jeffery G. SavenChapter 13 Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts (pages 343–362): Kevin K. Desai and Brian G. MillerChapter 14 Designing Programmable Protein Switches (pages 363–389): Martin SagermannChapter 15 The Cyclization of Peptides and Proteins with Inteins (pages 391–407): Blaise R. Boles and Alexander R. HorswillChapter 16 A Method for Rapid Directed Evolution (pages 409–439): Manfred T. ReetzChapter 17 Evolution of Enantioselective Bacillus subtilis Lipase (pages 441–451): Thorsten Eggert, Susanne A. Funke, Jennifer N. Andexer, Manfred T. Reetz and Karl?Erich JaegerChapter 18 Circular Permutation of Proteins (pages 453–471): Glenna E. Meister, Manu Kanwar and Marc OstermeierChapter 19 Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): A Versatile Tool for Generating Targeted Libraries (pages 473–480): Asael Herman and Dan S. TawfikChapter 20 Protein Engineering by Structure?Guided SCHEMA Recombination (pages 481–492): Gloria Saab?Rincon, Yougen Li, Michelle Meyer, Martina Carbone, Marco Landwehr and Frances H. ArnoldChapter 21 Chimeragenesis in Protein Engineering (pages 493–514): Manuela Trani and Prof. Dr. Stefan LutzChapter 22 Protein Generation Using a Reconstituted System (pages 515–535): Bei?Wen Ying and Takuya UedaChapter 23 Equipping in vivo Selection Systems with Tunable Stringency (pages 537–561): Martin Neuenschwander, Andreas C. Kleeb, Peter Kast and Donald HilvertChapter 24 Protein Engineering by Phage Display (pages 563–603): Agathe Urvoas, Philippe Minard and Patrice SoumillionChapter 25 Screening Methodologies for Glycosidic Bond Formation (pages 605–620): Amir Aharoni and Stephen G. WithersChapter 26 Yeast Surface Display in Protein Engineering and Analysis (pages 621–648): Benjamin J. Hackel and Dane WittrupChapter 27 In Vitro Compartmentalization (IVC) and Other High?Throughput Screens of Enzyme Libraries (pages 649–667): Amir Aharoni and Dan S. TawfikChapter 28 Colorimetric and Fluorescence?Based Screening (pages 669–711): Jean?Louis ReymondChapter 29 Confocal and Conventional Fluorescence?Based High Throughput Screening in Protein Engineering (pages 713–751): Ulrich Haupts, Oliver Hesse, Michael Strerath, Peter J. Walla and Wayne M. CocoChapter 30 Alteration of Substrate Specificity and Stereoselectivity of Lipases and Esterases (pages 753–775): Dominique Bottcher, Marlen Schmidt and Uwe T. BornscheuerChapter 31 Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering (pages 777–796): Matthew DeSieno, Jing Du and Huimin ZhaoChapter 32 Protein Engineering of Modular Polyketide Synthases (pages 797–827): Alice Y. Chen and Chaitan KhoslaChapter 33 Cyanophycin Synthetases (pages 829–848): Anna Steinle and Alexander SteinbuchelChapter 34 Biosynthetic Pathway Engineering Strategies (pages 849–876): Claudia Schmidt?Dannert and Alexander PisarchikChapter 35 Natural Polyester?Related Proteins: Structure, Function, Evolution and Engineering (pages 877–914): Seiichi Taguchi and Takeharu TsugeChapter 36 Bioengineering of Sequence?Repetitive Polypeptides: Synthetic Routes to Protein?Based Materials of Novel Structure and Function (pages 915–938): Sonha C. Payne, Melissa Patterson and Vincent P. ConticelloChapter 37 Silk Proteins – Biomaterials and Bioengineering (pages 939–959): Xiaoqin Wang, Peggy Cebe and David. L. Kaplan