دانلود کتاب Single Molecule Detection in Solution: Methods and Applications

The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years. This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy). In the second part, various applications in life sciences and medicine provide the latest research results. This modern handbook is a highly accessible reference for a broad community from advanced researchers, specialists and company professionals in physics, spectroscopy, biotechnology, analytical chemistry, and medicine. Written by leading authorities in the field, it is timely and fills a gap - up to now there exists no handbook concerning this theme. Content: Chapter 1 Single Molecule Detection in Liquids and on Surfaces under Ambient Conditions: Introduction and Historical Overview (pages 1–19): Jorg Enderlein, Richard A. Keller and Christoph ZanderChapter 2 Theoretical Foundations of Single Molecule Detection in Solution (pages 21–67): Jorg Enderlein and Christoph ZanderChapter 3 Conceptual Basis of Fluorescence Correlation Spectroscopy and Related Techniques as Tools in Bioscience (pages 69–120): Jerker Widengren and Ulo MetsChapter 4 Surface?Enhanced Raman Scattering (SERS) – A Tool for Single Molecule Detection in Solution (pages 121–144): Katrin Kneipp, Harald Kneipp, Irving Itzkan, Ramachandra R. Dasari and Michael S. FeldChapter 5 Single Molecule Detection on Surfaces with the Confocal Laser Scanning Microscope (pages 145–183): Martin Bohmer and Jorg EnderleinChapter 6 Spectroscopy of Individual Photosynthetic Pigment?Protein Complexes (pages 185–229): J. Wrachtrup, T.J. Aartsma, J. Kohler, M. Ketelaars, A. M. van Oijen, M. Matsushita, J. Schmidt, C. Tietz and F. JelezkoChapter 7 Single Dye Tracing for Ultrasensitive Microscopy on Living Cells (pages 231–245): Gerhard J. Schutz and Hansgeorg SchindlerChapter 8 Single Molecule Identification in Solution: Principles and Applications (pages 247–272): M. Sauer and C. ZanderChapter 9 Studying Molecular Motors on the Single Molecule Level (pages 273–292): Y. Ishii, A. H. Iwane, H. Yokota, Y. Inoue, T. Wazawa, M. Nishiyama, H. Tanaka, K. Kitamura and T. YanagidaChapter 10 The Chemistry of a Single Enzyme Molecule (pages 293–301): Robert Polakowski, Michael Eggertson, Douglas B. Craig and Norman J. DovichiChapter 11 Single Molecule Detection of Specific Nucleic Acid Sequences (pages 303–321): Alonso CastroChapter 12 Single Molecule Detection in the Near?Infrared (pages 323–362): Steven A. Soper, Musundi Wabuyele, Clyde V. Owens and Robert P. Hammer